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| Registros recuperados: 57 | |
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| Xavier,M.N.; Sant'Anna,F.M.; Silva,T.M.A.; Costa,E.A.; Moustacas,V.S.; Merlo,F.A.; Carvalho Júnior,C.A.; Dasso,M.G.; Mathias,L.A.; Gouveia,A.M.G.; Lage,A.P.; Santos,R.L.. |
| A infecção por Brucella ovis é considerada uma das principais causas de epididimite e infertilidade em carneiros, resultando em falhas reprodutivas e perdas econômicas significativas em rebanhos ovinos ao redor do mundo. O estudo teve o objetivo de avaliar três testes sorológicos disponíveis para o diagnóstico da brucelose ovina por B. ovis, utilizando 181 soros ovinos. Amostras de soro provenientes de carneiros experimentalmente infectados foram coletadas ao longo de 192 dias pós-infecção (n=117) e durante o período pré-infecção (n=9). Adicionalmente, amostras de soro foram obtidas de ovinos provenientes de um rebanho livre para B. ovis (n=55). As técnicas de imunodifusão em gel de agar (IDGA), utilizando dois antígenos disponíveis comercialmente, e de... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ram; Brucella ovis; Serology; Complement fixation; Immunodiffusion. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000400031 |
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| Langoni,H.; Lucheis,S. B.; Da Silva,R. C.; Castro,A. P. B.; Paes,A. C.. |
| Visceral leishmaniasis is a zoonotic disease caused by parasites of the Leishmania genus. Dog is the major source of infection to man, especially in urban areas. The authors report a case of visceral leishmaniasis in a pit bull female dog from Bocaina, São Paulo, Brazil. The animal presented clinical signs compatible with leishmaniasis, including skin lesions in the body and partial damage of the external ears. The indirect fluorescent antibody test (IFAT) demonstrated a titer of 1280, and promastigote forms of Leishmania sp were isolated by the culture of bone marrow puncture. Cytological analysis of the lymph node and smear of the bone marrow puncture revealed macrophages containing amastigote forms of Leishmania sp in their inner region. The test of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leishmania; Zoonosis; Dog; Diagnosis; Hemoculture; Serology; Polymerase chain reaction (PCR). |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000300012 |
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| Lunkes,Vinícius Leobet; Tonin,Alexandre Alberto; Machado,Gustavo; Corbellini,Luis Gustavo; Diehl,Gustavo Nogueira; Santos,Lucila Carboneiro dos; Bezerra,Camila de Sousa; Azevedo,Sérgio Santos de; Pequeno,Nebson Fernandes; Silva,Adriana Moraes da; Weiblen,Rudi; Flores,Eduardo Furtado. |
| ABSTRACT: Vesicular stomatitis virus (VSV) is the agent of a vesicular disease that affects many animal species and may be clinically confounded with foot-and-mouth disease in ruminant and swine. Horses are especially susceptible to VSV and may serve as sentinels for virus circulation. The present study investigated the presence of neutralizing antibodies against VSV Indiana III (VSIV-3) in serum samples of 3,626 horses from six states in three Brazilian regions: Southern (RS, n = 1,011), Midwest (GO/DF, n = 1,767) and Northeast (PB, PE, RN and CE, n = 848) collected between 2013 and 2014. Neutralizing antibodies against VSIV-3 (titers ≥40) were detected in 641 samples (positivity of 17.7%; CI95%:16.5-19.0%), being 317 samples from CE (87.3%; CI95%:... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Serology; Differential diagnosis; Foot-and-mouth disease; Zoonosis; Vesicular disease. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000801424 |
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| Bulos,L.H.S.; Gonzaga,N.F.; Souza,L.F.L.; Assao,V.S.; Leite,R.A.S.; Rebouças,M.S.; Scatamburlo,T.M.; Guimarães,J.D. Guimarães S.E.F.; Lopes,P.S.; Almeida,M.R.L.; Silva Júnior,A.. |
| ABSTRACT Brazilian pig population is made up of several naturalized breeds; among them the Piau breed is known for its rusticity and large fat stores. The naturalized breeds, in comparison with commercial ones, may have an increased resistance to diseases circulating in their territory. Thus, this study aimed to verify if there are differences between the serologic profile against Porcine circovirus 2 (PCV2) of Piau pigs and that of a commercial breed from a farm naturally infected by PCV2. The serum viral load was measured by qPCR, and levels of anti-PCV2 antibodies were measured by ELISA. The results showed that the serum viral load was similar across all animals. However, Piau piglets showed higher levels of antibodies compared to commercial piglets (P=... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Serum viral load; Serology; Piau. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000501145 |
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| Langoni,H.; Da Silva,A.V.; Pezerico,S.B.; De Lima,V.Y.. |
| Equine leptospirosis can present a non-symptomatic form, an acute clinical form, or even develop chronically, causing reproductive alterations, such as abortion and recurrent uveitis. Since the prevalence of leptospirosis in several countries and regions is widely reported, the objective of this study was to verify the prevailing equine leptospirosis in different regions of Brazil. Sera from 1402 blood samples from horses of different age, sex, breed, and purpose were examined. These samples came from southeastern and central west states of Brazil. The method utilized was the Microscopic Agglutination Test (MAT), with 12 different Leptospira serovars. From the sera tested, 754 (54%) were positive for one (385) or more (372) serovars. These results were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leptospira; Horse; Serology. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992004000300003 |
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| Lamas,C.; Favacho,A.; Ramos,R.G.; Santos,M.S.; Ferravoli,G.I.; Weksler,C.; Rozental,T.; Bóia,M.N.; Lemos,E.R.S.. |
| Bartonella is an important cause of blood culture-negative endocarditis in recent studies. Seroprevalence studies in the States of Minas Gerais and Rio de Janeiro have shown Bartonella IgG positivity around 14% in healthy adults and 40% in HIV seropositive adults, respectively. A case report of a 46-year-old white male with moderate aortic regurgitation (AR) due to rheumatic heart disease (RHD), admitted due to worsening heart failure, is presented. Clinical features were apyrexia, anemia, polyclonal hypergammaglobulinemia, hematuria and splenomegaly. He was submitted to surgery due to worsening AR. Histopathology of the excised valve showed active bacterial endocarditis and underlying RHD. Routine blood cultures were negative. Indirect immunofluorescence... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bartonella spp.; Infective endocarditis; Blood culture-negative infective endocarditis; Rheumatic heart disease; Serology; Indirect immunofluorescence. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000600012 |
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| Oliveira,GH; Berchieri Jr,A; Montassier,HJ. |
| This study evaluated two enzyme-linked immunosorbent assays (ELISA) in the detection of chicken serologic response against Salmonella enterica sorotype Typhimurium. The assays have used as detecting antigen the soluble bacterial proteins of a non-flagellated strain of Salmonella Typhimurium (AgTM), and antibody conjugated to peroxidase or alkaline phosphatase. According to the results, optimal dilutions of antigen (concentration 5.49 mg/mL) and serum samples in both assays were 1:20,000 and 1:1,000, respectively. In such conditions, the ELISA/AgTM was able to detect serological response to Salmonella Typhimurium. Cross-reactions to Salmonella serotypes Gallinarum and Pullorum were seen, but not with other serotypes such as Enteritidis. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chicken; ELISA; Enzyme; Salmonella sp; Serology. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2006000100008 |
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| Grisez, L.; Ollevier, F.P.. |
| The different serotyping systems, based on thermostable O antigens, reported for <i>Vibrio anguillarum</i> and <i>V. ordalii</i> were compared by quantitative agglutination, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and subsequent silver staining or Western blotting (immunoblotting) of purified lipopolysaccharide (LPS), using polyclonal rabbit antisera. The results demonstrate that 16 different serotypes within <i>V. anguillarum</i> (designated 01 to 016) can be distinguished. Each of these serotypes is characterized by a distinct polysaccharide banding pattern, as revealed by silver-stained gels of purified LPS. The comparative analysis allowed a complete alignment of the different stereotypes for the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pathogens; Serology; Vibrio anguillarum. |
| Ano: 1995 |
URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=2970 |
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| De Paula,Sérgio Oliveira; Fonseca,Benedito Antônio Lopes da. |
| Dengue is the most important disease caused by an arbovirus (1, 2, 3 and 4 serotypes) worldwide, especially in the tropical and sub-tropical regions. Its clinical manifestations range from asymptomatic infections to a severe disease characterized by hemorrhage and shock. The incidence of dengue virus activity in the Americas has substantially increased from 1980 to 1994. In Brazil, the increase in the incidence of dengue is especially linked to the dissemination of Aedes aegypti. Thus, a rapid and accurate dengue diagnosis is of paramount importance for effective control of dengue outbreaks [8]. Five serological tests have been used for the diagnosis of dengue infection: hemagglutination-inhibition (HI), complement fixation (CF), neutralization test (NT),... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dengue; Diagnosis; Serology; PCR. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702004000600002 |
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| Ruiz García, Noé. |
| La dispersión espacio-temporal del Citrus tristeza virus se caracterizó en Tamaulipas de diciembre de 2001 a marzo de 2004, en 11 huertos comerciales de naranja dulce injerta- dos en naranjo agrio. Adicionalmente, se comparó el desempeño de la técnica serológica inmunoimpresión-ELISA con respecto a DAS-ELISA propuesta en la normatividad ofi- cial mexicana de la Campaña Nacional contra CTV. El objetivo fue proporcionar bases epidemiológicas e incorporar criterios de diagnóstico viral en una propuesta de muestreo con fines de erradicación. Inmunoimpresión-ELISA fue tan confiable (97.8 %) como DAS-ELISA en identificar árboles positivos, siendo 54.9% más económica e implicó solo una sexta parte del tiempo de procesado. La detectabilidad en impresiones... |
| Tipo: Tesis |
Palavras-chave: Citrus tristeza virus; Serología; Muestreo; Vectores; Epidemiología Citrus tristeza virus; Serology; Sampling; Vectors; Epidemiology. |
| Ano: 2012 |
URL: http://hdl.handle.net/10521/1020 |
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| Ruiz García, Noé. |
| La dispersión espacio-temporal del Citrus tristeza virus se caracterizó en Tamaulipas de diciembre de 2001 a marzo de 2004, en 11 huertos comerciales de naranja dulce injerta- dos en naranjo agrio. Adicionalmente, se comparó el desempeño de la técnica serológica inmunoimpresión-ELISA con respecto a DAS-ELISA propuesta en la normatividad ofi- cial mexicana de la Campaña Nacional contra CTV. El objetivo fue proporcionar bases epidemiológicas e incorporar criterios de diagnóstico viral en una propuesta de muestreo con fines de erradicación. Inmunoimpresión-ELISA fue tan confiable (97.8 %) como DAS-ELISA en identificar árboles positivos, siendo 54.9% más económica e implicó solo una sexta parte del tiempo de procesado. La detectabilidad en impresiones... |
| Tipo: Tesis |
Palavras-chave: Citrus tristeza virus; Serología; Muestreo; Vectores; Epidemiología; Doctorado; Fitopatología; Fitopatología; Serology; Sampling; Vectors; Epidemiology. |
| Ano: 2008 |
URL: http://hdl.handle.net/10521/1348 |
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| Barreto,Maria Lucia; Nascimento,Elmiro Rosendo do; Campos,Carlos Augusto de Martino; Nascimento,Maria da Graça Fichel do; Lignon,Gilberto Brasil; Lira,Marie Luce Flores; Silva,Ricardo G.. |
| This work was conducted on rats in two premises located in Niterói and Rio de Janeiro, Brazil. One is classified as conventional controlled and the other, conventional. The objective of the present study was to detect the presence of Mycoplasma pulmonis in animals with symptoms of respiratory disease and low reproductive performance. In the conventional controlled premises, 16 rats of Wistar-Furth strain were necropsied while in the conventional premises necropsy was performed on 12 rats of Hooded Lister strain. The clinical samples of lungs, trachea, oropharynx, middle ear, uterus and ovaries were subjected to culturing while the sera were tested for antibody detection. From 28 rats, 57.14% (16/28) were culture positive for M. pulmonis, being 81.25%... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mycoplasma pulmonis; Diagnosis; Isolation; Serology; Rats. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000300015 |
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| Álvarez,Elba; Iglesia,Aleika. |
| Information about the characterization and diagnostic of wild fire was compiled. This bacterial disease, exotic for Cuba, is caused by Pseudomonas syringae pv. tabaci. The species Pseudomonas syringae is traditionally divided into pathovars depending on the host range and symptoms. For its study, different phenotypic and genotypic methods are used. Such methods allow the traditional taxonomic confirmation of this pathovar within the species. Serological and molecular techniques such as: ELISA tests, immunofluorescence, polymerase chain reaction (PCR) are also used for the characterization and detection of the bacterium. Such techniques are very useful and sensible for the diagnostic. |
| Tipo: Journal article |
Palavras-chave: Pseudomonas syringae pv. tabaci; Rep-PCR; Pathogenicity; Serology. |
| Ano: 2007 |
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522007000300001 |
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| Registros recuperados: 57 | |
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